We offer a list with hundreds of 7G One® PP Mix with designed sequences of primer-probe sets to test the target pathogens for animals, plants, and humans.,and each sequence set would be named one sequence number(S/N). If our listed sequences cannot meet your needs, you can prepare your own 7G One® PP Mix with 4×7G One® PP Mix-IPC and your own primer-probe sets.
7G One® PP Mix is a POCT brick specially designed for "Dropper PCR", which can be easily set up a qPCR reaction with the Self-Collection Tube and 7G One® EnMix bricks. During the use process, it does not rely on professional personnel,pipettes and any instruments. The dropper bottle contains a primer-probe mix with 1 or 2 Target Genes and IPC gene. The probe of external internal positive control (IPC) is labeled with ROX and the amplification curve is yellow, which can monitor the whole procedures including reagents, operation,inhibitor and qPCR cycler, to avoid false negative results.The probe of target gene probe is labeled with VIC or FAM, and the amplification curve is green of VIC or blue of FAM. Using the POCT bricks, target gene of a certain pathogen or gene can be quickly detected with 1-step RT-qPCR technology.
We offer a list with hundreds of 7G One® PP Mix with designed sequences of primer-probe sets to test the target pathogens for animals, plants, and humansas follows，and each sequence set would be named one sequence number(S/N).
If our listed sequences cannot meet your needs, you can prepare your own 7G One® PP Mix with 4×7G One® PP Mix-IPC and your own primer-probe sets as follows.
-Preparation of 7G One® PP Mix in a dropper bottle :The recommended final concentration is 0.4–0.8 µM for primers and 0.1 µM for probes. If detection sensitivity is insufficient, please adjust the concentrations. Add 300 µL of 4×primer-probe premix to the 4×7G One® PP Mix-IPC(S/N:IPC300）and mix thoroughly to prepare the 7G One® PP Mix. 
Cut open the 8-strips tubes and caps of the 7G One® EnzMix according to the required quantity.
Remove the transparent dropper cap of the dropper bottle with the template and drop 1 drop into the tubes directly.
Remove the transparent dropper cap of the dropper bottle with the 7G One® PP Mix and drop 1 drop into the tubes.
Please cover the test tube cap and lightly flick the tube wall. Fully mix and place it in the qPCR cycler.
This reagent contains 600uL of primer-probe mix with 1 or 2 Target Genes and IPC gene，and at least 16 drops can be dripped in dropper PCR protocol.
This reagent can be stored at 2-8°C for 2 years and protected from light.
Manual：7G One ® PP Mix- Flyer：Listed items p primer-probe sets to test the target pathogen.
- COA： Certificate of Analysis for 7G1-016
Catalog Number | Product Name | Unit Size | Price |
7G1-016 | 600 µL/ bottle | US$ 12.00-US$ 20.00 |