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RNA Dilution&Storage Solution

RNA Solution

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RNA Dilution&Storage Solution is a buffer that provides greater RNA stability than TE Buffer or RNase-free water. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution.RNA Dilution&Storage Solution has two features that keeps RNA stable: a low pH of 7.0, and a Chemical RNase Inhibitor. RNA Dilution&Storage Solution Solution is compatible with all common RNA applications such as reverse transcription, RT-PCR,   1- step RT-qPCR, in vitro transcription, northern analysis, and nuclease protection assays.
TOROBlue® qRT Premix with gDNA Eraser

qRT Kit

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TOROBlue® qRT Premix with gDNA Eraser enables fast cDNA synthesis for 2-step RT-qPCR and includes a rapid step that eliminates genomic DNA contamination without loss of input RNA. Genomic DNA is eliminated by treating the RNA templates with gDNA Eraser Mix for 2-5 minutes at 37°C. 5×qRT Premix contains  primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for qPCR. The protocol is simple, and the reaction can be completed in 10-25 min.
TOROIVD® Probe 1-step RT-qPCR 5G kit 2.0

PCR&RT Enzymes

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TOROIVD®Probe 1-step RT-qPCR 5G kit 2.0 provides for sensitive, reproducible detection up to five RNA and DNA targets in a single multiplex reaction.Particularly useful for virus detection with TaqMan® probe assays, the kit includes UNG , dsDNase ,  thermostable MMLV reverse transcriptase, RNase inhibitor, TOROIVD®5G DNA polymerase and reaction buffer.  The improved enzymes also enables a high resistance to PCR inhibitors and high stability in room temperature. The 1-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination with Uracil-N-glycoslyase（UNG）and dsDNase. The kit is suitable for high-speed RT-qPCR and enables accurate detection and quantification of targets, making it possible to obtain highly reproducible and reliable realtime PCR results over a wide dynamic range.
TOROGreen® HRM qPCR Master Mix

HRM qPCR Mix

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TOROGreen® HRM qPCR Master Mix is a Taq DNA polymerase based 2 × master mix for use in qPCR applications and high-resolution melting (HRM) analysis, which contains the HotStart Taq DNA Polymerase, PCR Buffer, dNTPs, Super EvaGreen® dye, ROX, Enhancer and Stabilizer. Hot Start technology with anti -Taq DNA polymerase antibodies enableshigh specificity and reproducible amplification.
TOROIVD® 5G DNA polymerase

PCR&RT Enzymes

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TOROIVD® 5G DNA polymerase is an engineered Tth DNA polymerase for improved processivity,sensitivity and inhibitor tolerance through a process of directed evolution.The anti-Tth DNA polymerase antibodies was used in the enzyme solution to inhibit polymerase activity for Hot Start PCR. Like wild-type Tth DNA polymerase, The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→ 3’ exonuclease activity in the presence of Mn2+ ions. Therefore, this enzyme also enables "1-step RT-qPCR" including the reverse transcription and PCR steps.The enzyme is supplied with three reaction buffers, qPCR Buffer GB , qPCR Buffer T and RT-qPCR Buffer R.  2×5G qPCR Buffer GB is specifically formulated for  high sensitivity qPCR or 1-step RT-qPCR with MMLV Reverse transcriptase, while  2×5G qPCR Buffer T is recommended when template samples are contaminated with PCR inhibitors. 2×5G RT-qPCR Buffer R is recommended for 1-step RT-qPCR in the presence of Mn2+ ions.
TOROIVD® III Reverse Transcriptase

PCR&RT Enzymes

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TOROIVD® III Reverse Transcriptase is an engineered of M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase  with removed RNase H activity  to increase reaction efficiency thermal activity.  The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C, providing increased specificity, higher yields of cDNA, and more full length product than other reverse transcriptases. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.

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