【Description】

• TOROGreen^® 5G qPCR Premix 2.0 is an 2×master mix for intercalator-based realtime PCR with SYBRGreen I, which contains all components except for the primer. TOROIVD^® 5G DNA polymerase, a mutant hot-start Tth DNA polymerase modified by antibodies, allows high specificity and sensitivity for high-speed PCR reactions. The improved polymerase and reaction mixture combination also enables a high resistance to PCR inhibitors. ROX is added into the premix and can be applied to the qPCR cyclers that require a passive reference dye. The premix is suitable for high-speed PCR and enables accurate detection and quantification of targets, making it possible to obtain highly reproducible and reliable realtime PCR results over a wide dynamic range.

【Feature】

• -Rapid and highly sensitive

  This premix can achieve the rapid and highly sensitive quantification of a low-copy targets and be suitable for the quantification of DNA viruses or cDNA at a low level.

• -Room-temperature stable: 

  The specially optimized PCR buffer make the mix very stable at room temperature.Therefore, the performance is not easily decrease during storing and shipping.

• -Inhibitor tolerant

  The unique proprietary formulation of this kit allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA.

• -Wide dynamic range

  The master mix demonstrates excellent reproducibility over a wide dynamic range and provides efficient amplification over 8 logs of sample input.

【Components】

•  QST-200 can be used for 1000 reactions for a total 20µL reaction volume.

• Cat NO.	Components	Size
  QST-200	TOROGreen®5G qPCR Premix 2.0	1.25 mL ×8 tubes/kit

  
  

  
  

  
  

【Storage】

• This reagent can be stored at 2-8°C for 24 months and protected from light. 

• For longer storage, this reagent should be kept at -20°C. 

【Primer Design】

• - Primer length: 18~30bp  - GC content of primer: 40~60% -Target length: ≤ 200 bp (optimally, ≤ 150bp)

• -Checking the primers:

  -NTC tests can distinguish unintended amplification products of primer dimers  from the intended PCR products in SYBR Green I reactions. NTC test is required to verify the each of primers for assessing the extent of primer dimers.The primer of NTC with Cq<40 should be redesigned. 

  -Prepare a dilution series with five or more dilutions of template DNA. Perform qPCR assay using the diluted DNA with the newly designed primers and draw a standard curve. Confirm that the PCR efficiency is between 95% and 105% and R2 is equal to or greater than 0.99. If the PCR efficiency or R2 are outside of these ranges, the primers concentration and reaction conditions should be optimized. If this does not improve the result, the primers should be redesigned.

【References】

• Bustin SA, Benes V, Garson JA, etc,al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.ClinChem.2009,Apr;55(4):611-22.

【Documents】

•     Manuals:   TOROGreen®5G qPCR Premix 2.0 (QST-200)- English-

•     Brochures:   Real-time PCR Premix for gene expression analysis

•     MSDS： Material Safety Data Sheet for QST-200

•     COA： Certificate of Analysis for QST-200 

    

【Order information】