TOROIVD

Evolving Enzymes

Inovating IVD

Evolving Enzymes

Inovating IVD

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Focus On Life Science&IVD

About US

TOROIVD is an evolving international company, which develops, manufactures and markets a wide range of specialized bioresearch reagents and IVD products that simplify, accelerate and improve life sciences research and clinical diagnosis . Our vision is to position TOROIVD at the forefront of human, animal and plant health through continuous innovation.

Founded in 2017 and headquartered in China, TOROIVD is an evolving international company and our mission is to provide customers with a range of products which are fast and easy to use, ……

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News Center

Weekly epidemiological update on COVID-19 - 12 April 2022

2022-04-14
"Test-tube"evolution wins Chemistry Nobel Prize

2018-10-07
Syphilis cases in US newborns spike to 20-year high

2018-10-02
Cancer immunologists scoop medicine Nobel prize

2018-10-02

FAQ

TOROIVD® Probe 1-step RT-qPCR 5G kit 2.0

PCR&RT Enzymes

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TOROIVD®Probe 1-step RT-qPCR 5G kit 2.0 provides for sensitive, reproducible detection up to five RNA and DNA targets in a single multiplex reaction.Particularly useful for virus detection with TaqMan® probe assays, the kit includes UNG , dsDNase ,  thermostable MMLV reverse transcriptase, RNase inhibitor, TOROIVD®5G DNA polymerase and reaction buffer.  The improved enzymes also enables a high resistance to PCR inhibitors and high stability in room temperature. The 1-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination with Uracil-N-glycoslyase（UNG）and dsDNase. The kit is suitable for high-speed RT-qPCR and enables accurate detection and quantification of targets, making it possible to obtain highly reproducible and reliable realtime PCR results over a wide dynamic range.
TOROGreen® HRM qPCR Master Mix

HRM qPCR Mix

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TOROGreen® HRM qPCR Master Mix is a Taq DNA polymerase based 2 × master mix for use in qPCR applications and high-resolution melting (HRM) analysis, which contains the HotStart Taq DNA Polymerase, PCR Buffer, dNTPs, Super EvaGreen® dye, ROX, Enhancer and Stabilizer. Hot Start technology with anti -Taq DNA polymerase antibodies enableshigh specificity and reproducible amplification.
TOROIVD® 5G DNA polymerase

PCR&RT Enzymes

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TOROIVD® 5G DNA polymerase is an engineered Tth DNA polymerase for improved processivity,sensitivity and inhibitor tolerance through a process of directed evolution.The anti-Tth DNA polymerase antibodies was used in the enzyme solution to inhibit polymerase activity for Hot Start PCR. Like wild-type Tth DNA polymerase, The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→ 3’ exonuclease activity in the presence of Mn2+ ions. Therefore, this enzyme also enables "1-step RT-qPCR" including the reverse transcription and PCR steps.The enzyme is supplied with three reaction buffers, qPCR Buffer GB , qPCR Buffer T and RT-qPCR Buffer R.  2×5G qPCR Buffer GB is specifically formulated for  high sensitivity qPCR or 1-step RT-qPCR with MMLV Reverse transcriptase, while  2×5G qPCR Buffer T is recommended when template samples are contaminated with PCR inhibitors. 2×5G RT-qPCR Buffer R is recommended for 1-step RT-qPCR in the presence of Mn2+ ions.
TOROIVD® III Reverse Transcriptase

PCR&RT Enzymes

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TOROIVD® III Reverse Transcriptase is an engineered of M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase  with removed RNase H activity  to increase reaction efficiency thermal activity.  The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C, providing increased specificity, higher yields of cDNA, and more full length product than other reverse transcriptases. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.
TOROIVD® Probe 1-step RT-qPCR 5G kit

Probe qPCR Mix

List price:￥0.00

Price:￥0.00

TOROIVD® Probe 1-step RT-qPCR 5G kit provides for sensitive, reproducible detection up to five RNA/DNA targets in a single multiplex reaction .Particularly useful for virus detection with TaqMan®probe assays , the kit includes UNG, thermostable MMLV reverse transcriptase, RNase inhibitor ,TOROIVD® 5G DNA polymerase and reaction buffer.  The improved enzymes also enables a high resistance to PCR inhibitors and high stability in room temperature. The 1-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination with uracil-N-glycoslyase（UNG） . The kit is suitable for high-speed RT-qPCR and enables accurate detection and quantification of targets, making it possible to obtain highly reproducible and reliable realtime PCR results over a wide dynamic range.