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   Inovating IVD

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TOROBlue® One HS KOD Kit
    Publish time 2019-09-15 08:40    

 

 

TOROBlue® One HS KOD Kit  includes 2× One HS KOD Mix with dye is  a  ready-to-use premixed solution containing genetically modified hotstart KOD DNA polymerase, PCR buffer, dNTPs,elongation accelerator and loading dye.This mix which enables fast PCR, which has an extension time of  5 sec/ kb  by applying  genetically modified  KOD DNA polymerase and a new elongation accelerator. In addition, this mix  provides  greater  efficiency  and elongation capabilities than conventional PCR enzymes. In particular, these show greater amplification success from crude specimens.

DESCRIPTION

 

TOROBlue® One HS KOD Kit  includes 2× One HS KOD Mix with dye is  a  ready-to-use premixed solution containing genetically modified hotstart KOD DNA polymerase, PCR buffer, dNTPs,elongation accelerator and loading dye.This mix which enables fast PCR, which has an extension time of  5 sec/ kb  by applying  genetically modified  KOD DNA polymerase and a new elongation accelerator. In addition, this mix  provides  greater  efficiency  and elongation capabilities than conventional PCR enzymes. In particular, these show greater amplification success from crude specimens.

 

FEATURES


-Fast: can amplify the targets using the following very short conditions:0~ 10 kb: 5 sec/ kb ; ≥ 10 kb: 10 sec/ kb

-Easy-to-use: premixed with loading dye for directly loading the gel of Post-Staining or Precasting .

-High Fidelityexhibits approximately 80-fold higher fidelity than Taq DNA polymerase for cloning.

-Direct PCR: effective for amplification from crude samples.


APPLICATION


-Direct PCR

-Colony PCR

-Amplification of NGS libraries

-Site direct gene mutation


COMPONENTS


The kit includes the following  reagents,  which can be used for 40 reactions for a total 50ul reaction volume,or 100 reactions for a total 20ul reaction volume.

Cat No. :KAO-201  

2× One HS KOD Mix  with Dye                      1ml ×1 tube


PROTOCOL

1.Standard reaction setup

(1)Before preparing the mixture, all components should be completely thawed on ice.


 


(2)Gently mix the reaction solutions and spin down in microcentrifuge.  


Notes:

-Primers should be 22-35 bases with  Tm ≥ 65°C.

-Optimal primer concentration is 0.3uM. In the case of long targets (≥10 kb), reduced primers concentration (0.15uM) may give more effective amplification.

-When PCR yield is low, increased primers concentration (0.5 uM) may give more effective amplification.

-Decreased the amount of template DNA when no PCR Product.   

    

2.Cycling conditions


 


Notes:

- Extension time:0-10kb,5sec/kb; ≥ 10 kb,10sec/kb.

-Longer extension time may enhance efficiency. For amplification from a

low copy DNA or crude sample, the extension time should be 10 sec. /kb.

-Poor amplification may be improved by changing the denaturation step

to 94°C, 15sec.   

 

3.Electrophoresis


(1)Directly  loading the PCR products and the suitable TOROBlue® Loading Marker  into the Post-Staining or Precasting agarose gel.

(2)Run on agarose electrophoresis to detect PCR products and marker.

 

STORAGE
   

  This reagent  his reagent can be stored at 4°C for 1 months. For longer storage,  this reagent should be kept at -20°C.





Ordering Information



Cat.No.

Product Name

Size

Price

Data Sheet

KAO-201


TOROBlue® One HS KOD Kit


50ul×40Reactions

US$  100.00


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