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TOROBlue® One HS Taq Kit
    Publish time 2019-09-14 16:13    

 

TOROBlue® One HS Taq Kit includes two components,2× One HS Taq Mix and TOROBlue® 6×DNA Loading Buffer. 2× One HS Taq Mix is a ready-to-use premixed solution containing hotstart Taq DNA polymerase, PCR buffer, dNTPs and PCR enhancer. This mix contains all components for PCR except primers and template DNA and shows specific and efficient amplification. 1 volume of TOROBlue® 6×DNA loading buffer  premixed with 5 volumes of  PCR products can be directly loaded into the  post-staining or precasting agarose gel.

DESCRIPTION


TOROBlue® One HS Taq Kit includes two components,2× One HS Taq Mix and TOROBlue® 6×DNA Loading Buffer. 2× One HS Taq Mix is a ready-to-use premixed solution containing hotstart Taq DNA polymerase, PCR buffer, dNTPs and PCR enhancer. This mix contains all components for PCR except primers and template DNA and shows specific and efficient amplification. 1 volume of TOROBlue® 6×DNA loading buffer  premixed with 5 volumes of  PCR products can be directly loaded into the  post-staining or precasting agarose gel.


FEATURES

 

- Enables greater PCR performance than conventional Taq DNA polymerase.

-This reagent contains anti-Taq antibodies for hot start PCR. Hot start technology realizes highly specific and sensitive PCR.

-Easy-to-use: offer TOROBlue® 6×DNA loading buffer premixed with premixed with  5 volumes of PCR products can be directly loaded into the gel.


APPLICATION


 -General PCR products for TA cloning.

 -Colony direct PCR

 -Multiplex PCR for SNPs etc.

 -High-throughput PCR.


COMPONENTS


The kit includes the following reagents, which can be used for 200 reactions for a total 50ul reaction volume, or 500 reactions for a total 20ul reaction volume.

Cat No. :TAO-200

2× One HS Taq Mix                                        1ml ×5tube

TOROBlue® 6×DNA loading buffer                  1ml ×2 tube


PROTOCOL


1.Standard reaction setup


(1)All components should be completely thawed before preparing the mixture at room temperature.


 


(2)Gently mix the reaction solutions and spin down in microcentrifuge.      


2.Cycling conditions


  

3.Electrophoresis


(1)Add 1 volume of TOROBlue®  6×DNA loading buffer to 5 volumes of PCR products.

(2) Mix well, spin down and load the mixture and the suitable TOROBlue® Loading Marker.

(3)Directly  loading the PCR products and the suitable TOROBlue® Loading Marker  into the post-staining or precasting agarose gel.

(4)Run on agarose electrophoresis to detect PCR products and marker.


STORAGE


     This reagent  his reagent can be stored at 4°C for 1 months. For longer storage,  this reagent should be kept at -20°C.



Ordering Information

Cat.No.

Product Name

Size

Price

Data Sheet

TAO-200

TOROBlue® One HS Taq Kit

50ul×200Reactions

US$  100.00


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